Coding

Part:BBa_K2132001:Design

Designed by: Shijie Gu   Group: iGEM16_ShanghaitechChina   (2016-08-26)


CsgASpyCatcherHisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part already includes the signal peptide for the secretion of CsgA. Also, remember, this part has two His tags.


Source

CsgA comes from the genomic sequence of the E. Coli. We obtained the sequence of CsgA from the Timothy K. Lu lab.

References