Coding
Part:BBa_K2132001:Design
Designed by: Shijie Gu Group: iGEM16_ShanghaitechChina (2016-08-26)
CsgASpyCatcherHisTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 841
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part already includes the signal peptide for the secretion of CsgA. Also, remember, this part has two His tags.
Source
CsgA comes from the genomic sequence of the E. Coli. We obtained the sequence of CsgA from the Timothy K. Lu lab.